NEETZoologyBiotechnology and Its Applications
Match List I with List II. List I List II (A) Pro-insulin (I) Prepared two DNA sequences corresponding to A and B chains (B) Mature insulin (II) Contains an extra stretch called C-peptide (C) Eli Lilly (III) Interconnects A and B polypeptides (D) Disulphide bonds (IV) Consists of only A and B chains Choose the correct answer from the options given below:
Options
- A(A)-(IV), (B)-(II), (C)-(I), (D)-(III)
- B(A)-(II), (B)-(IV), (C)-(III), (D)-(I)
- C(A)-(IV), (B)-(II), (C)-(III), (D)-(I)
- D(A)-(II), (B)-(IV), (C)-(I), (D)-(III)
Correct answer
D. (A)-(II), (B)-(IV), (C)-(I), (D)-(III)
Step-by-step solution
Pro-insulin is synthesized as a pro-hormone which contains an extra stretch called C-peptide. This matches (A) with (II). Mature insulin consists of only two short polypeptide chains: chain A and chain B. This matches (B) with (IV). In 1983, Eli Lilly, an American company, prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. This matches (C) with (I). Disulphide bonds interconnect the A and B polypeptides in mature insulin.