During the amplification of a gene of interest using PCR, a researcher sets the thermocycler for 5 continuous cycles. Assuming perfect efficiency, how many copies of the target DNA will be produced from a single

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Quantrex Academy · Accepted Answer

Correct answer: C. 32

In Polymerase Chain Reaction (PCR), the amount of DNA doubles after each cycle.

The number of DNA copies produced after

n

cycles is given by the formula

2^n \times N_0

, where

N_0

is the initial number of DNA molecules.

Given

n = 5

and

N_0 = 1

.

Number of copies

= 2^5 \times 1 = 32

.

Answer: 32

During the amplification of a gene of interest using PCR, a...

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