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NEET2026BiologyChapterActual

Which of the following statements regarding the enzymes used in recombinant DNA technology is incorrect?

Options

  1. ACellulase is used to degrade the cell wall of plant cells for isolating the genetic material.
  2. BExonucleases make cuts at specific positions within the DNA to produce fragments.
  3. CDNA ligase is utilized to link the alien gene of interest with the cloning vector.
  4. DProtease is added to the cellular extract to remove proteins during DNA purification.

Correct answer

B. Exonucleases make cuts at specific positions within the DNA to produce fragments.

Step-by-step solution

Exonucleases remove nucleotides from the ends of the DNA molecule. Endonucleases make cuts at specific positions within the DNA to produce fragments. Cellulase is used to break down the cell wall of plant cells to release the genetic material. DNA ligase acts as a molecular glue to join the alien DNA fragment with the plasmid vector. Protease is used during the isolation of DNA to degrade and remove proteins from the cellular extract. Answer: Exonucleases make cuts at specific positions within the DNA to produce fr

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