Read the following statements regarding the 'origin of replication' ( ori ) in recombinant DNA technology: A. It is a specific DNA sequence responsible for initiating replication in a chromosome or a vector. B. An alien piece of DNA can independently replicate in a host cell without being linked to this sequence. C. It is responsible for controlling the copy number of the linked alien DNA. D. It is the specific seque

Step-by-step solution

Statement A is correct because the origin of replication ( ori ) is a specific sequence of DNA where replication initiates. Statement B is incorrect because an alien piece of DNA cannot replicate independently in a host cell; it must be linked to the ori sequence to undergo replication. Statement C is correct as the ori sequence also controls the copy number of the linked alien DNA. Statement D is incorrect because restriction endonucleases bind and cleave at specific recognition sequences (palindromic nucleotide s