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NEET 2026
Exam
NEET
Year
2026
Subject
Biology
Chapter
Chapter

NEET 2026 · Biology · Chapter

During recombinant DNA procedures, the restriction endonuclease...

During recombinant DNA procedures, the restriction endonuclease EcoRI is widely used to cut DNA molecules. Which of the following correctly represents the specific palindromic nucleotide sequence recognized by this enzyme?

Options

  1. A5′−AAGCTT−3′5' - \text{AAGCTT} - 3'
    3′−TTCGAA−5′3' - \text{TTCGAA} - 5'
  2. B3′−GAATTC−5′3' - \text{GAATTC} - 5'
    5′−CTTAAG−3′5' - \text{CTTAAG} - 3'
  3. C5′−GAATTC−3′5' - \text{GAATTC} - 3'
    3′−CTTAAG−5′3' - \text{CTTAAG} - 5'
  4. D5′−GGATCC−3′5' - \text{GGATCC} - 3'
    3′−CCTAGG−5′3' - \text{CCTAGG} - 5'

Correct answer

C. 5′−GAATTC−3′5' - \text{GAATTC} - 3'
3′−CTTAAG−5′3' - \text{CTTAAG} - 5'

Step-by-step solution

The restriction endonuclease EcoRI recognizes the specific palindromic nucleotide sequence 5′−GAATTC−3′5' - \text{GAATTC} - 3' and 3′−CTTAAG−5′3' - \text{CTTAAG} - 5'.

The sequence 5′−AAGCTT−3′5' - \text{AAGCTT} - 3' is recognized by HindIII.

The sequence 5′−GGATCC−3′5' - \text{GGATCC} - 3' is recognized by BamHI.

Answer: 5′−GAATTC−3′5' - \text{GAATTC} - 3'
3′−CTTAAG−5′3' - \text{CTTAAG} - 5'
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