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NEET2026BiologyChapterActual

During recombinant DNA procedures, the restriction endonuclease EcoRI is widely used to cut DNA molecules. Which of the following correctly represents the specific palindromic nucleotide sequence recognized by this enzyme?

Options

  1. A5' - AAGCTT - 3' 3' - TTCGAA - 5'
  2. B3' - GAATTC - 5' 5' - CTTAAG - 3'
  3. C5' - GAATTC - 3' 3' - CTTAAG - 5'
  4. D5' - GGATCC - 3' 3' - CCTAGG - 5'

Correct answer

C. 5' - GAATTC - 3' 3' - CTTAAG - 5'

Step-by-step solution

The restriction endonuclease EcoRI recognizes the specific palindromic nucleotide sequence 5' - GAATTC - 3' and 3' - CTTAAG - 5' . The sequence 5' - AAGCTT - 3' is recognized by HindIII. The sequence 5' - GGATCC - 3' is recognized by BamHI. Answer: 5' - GAATTC - 3' 3' - CTTAAG - 5'

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