NEET2026BiologyChapterActual
In the E. coli cloning vector pBR322, the rop gene is responsible for:
Options
- Aproviding resistance against the antibiotic ampicillin.
- Bsynthesizing the enzyme -galactosidase for blue-white screening.
- Cacting as a recognition site for specific restriction endonucleases.
- Dcoding for proteins involved in the replication of the plasmid.
Correct answer
D. coding for proteins involved in the replication of the plasmid.
Step-by-step solution
In the E. coli cloning vector pBR322, the rop gene codes for the proteins involved in the replication of the plasmid. The amp^ R and tet^ R genes provide resistance against the antibiotics ampicillin and tetracycline, respectively. The ori sequence is responsible for initiating replication, while the rop gene product regulates the copy number of the plasmid by participating in the replication process. Answer: coding for proteins involved in the replication of the plasmid.