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NEET 2026
Exam
NEET
Year
2026
Subject
Biology
Chapter
Chapter

NEET 2026 · Biology · Chapter

A foreign DNA fragment was ligated at the BamH I restriction site...

A foreign DNA fragment was ligated at the BamH I restriction site of the pBR322 cloning vector. The resulting
A foreign DNA fragment was ligated at the BamH I restriction site of the pBR322 cloning vector. The resulting recombinant plasmids were introduced into host E. coliE.\ coli cells. The selection of recombinants was carried out as per the given flowchart.

Which of the following correctly represents the growth behavior of the recombinant transformants during this selection process?

Options

  1. AThey will form colonies on Plate 1 but will not survive on Plate 2.
  2. BThey will form colonies on both Plate 1 and Plate 2.
  3. CThey will not survive on either Plate 1 or Plate 2.
  4. DThey will not survive on Plate 1 but will form colonies on Plate 2.

Correct answer

A. They will form colonies on Plate 1 but will not survive on Plate 2.

Step-by-step solution

The pBR322 cloning vector contains two antibiotic resistance genes: ampicillin resistance (ampRamp^R) and tetracycline resistance (tetRtet^R).

The BamH I restriction site is located within the tetracycline resistance gene (tetRtet^R). When a foreign DNA fragment is ligated at the BamH I site, it disrupts the tetRtet^R gene, leading to its insertional inactivation. As a result, the recombinant plasmids lose their resistance to tetracycline but retain their resistance to ampicillin.

When these recombinant plasmids are introduced into host E. coliE.\ coli cells, the resulting recombinant transformants will be resistant to ampicillin but sensitive to tetracycline.

In the given selection process:
- Plate 1 contains an ampicillin medium. The recombinant transformants will grow and form colonies here because they possess the intact ampRamp^R gene.
- Plate 2 contains a tetracycline medium. The recombinant transformants will not survive here because their tetRtet^R gene has been inactivated.

Therefore, the recombinant transformants will form colonies on Plate 1 but will not survive on Plate 2.

Answer: They will form colonies on Plate 1 but will not survive on Plate 2.
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