NEET 2026
- Exam
- NEET
- Year
- 2026
- Subject
- Biology
- Chapter
- Chapter
NEET 2026 · Biology · Chapter
During the biochemical characterization of Griffith's transforming...
During the biochemical characterization of Griffith's transforming principle, it was observed that the transformation of non-virulent R strain bacteria into virulent S strain was inhibited by the addition of which of the following enzymes?
Options
- AProteases
- BRNases
- CDNases
- DLipases
Correct answer
C. DNases
Step-by-step solution
Avery, MacLeod, and McCarty performed the biochemical characterization of Griffith's transforming principle.
They purified biochemicals such as proteins, DNA, and RNA from heat-killed S cells to determine which molecule could transform live R cells into S cells.
They observed that protein-digesting enzymes (proteases) and RNA-digesting enzymes (RNases) did not affect the transformation of the non-virulent R strain into the virulent S strain.
However, digestion with DNase inhibited the transformation, proving that DNA was the transforming principle.
Therefore, the addition of DNases inhibited the transformation.
Answer: DNases
They purified biochemicals such as proteins, DNA, and RNA from heat-killed S cells to determine which molecule could transform live R cells into S cells.
They observed that protein-digesting enzymes (proteases) and RNA-digesting enzymes (RNases) did not affect the transformation of the non-virulent R strain into the virulent S strain.
However, digestion with DNase inhibited the transformation, proving that DNA was the transforming principle.
Therefore, the addition of DNases inhibited the transformation.
Answer: DNases