NEET2026BiologyChapterActual
In the process of isolating DNA for recombinant DNA technology, which of the following enzymes is specifically used to break open fungal cells?
Options
- ALysozyme
- BCellulase
- CChitinase
- DRibonuclease
Correct answer
C. Chitinase
Step-by-step solution
In recombinant DNA technology, the isolation of DNA requires breaking open the cells to release the genetic material along with other macromolecules. The cell wall of fungi is primarily composed of chitin. Therefore, the enzyme chitinase is specifically used to break open fungal cells. Lysozyme is used for bacterial cells, as their cell walls are made of peptidoglycan. Cellulase is used for plant cells, as their cell walls are made of cellulose. Ribonuclease is used to degrade RNA during the purification of DNA. An