NEET 2026
- Exam
- NEET
- Year
- 2026
- Subject
- Biology
- Chapter
- Chapter
NEET 2026 · Biology · Chapter
Given below are the key steps involved in the isolation of genetic...
Given below are the key steps involved in the isolation of genetic material from a fungal cell. Arrange these steps in the correct sequence.
A. Addition of chilled ethanol to the purified mixture.
B. Treatment of the cellular extract with RNase and Protease.
C. Breaking open the cell using the enzyme chitinase.
D. Collection of the precipitated DNA threads by spooling.
Choose the correct answer from the options given below:
A. Addition of chilled ethanol to the purified mixture.
B. Treatment of the cellular extract with RNase and Protease.
C. Breaking open the cell using the enzyme chitinase.
D. Collection of the precipitated DNA threads by spooling.
Choose the correct answer from the options given below:
Options
- AC, A, B, D
- BB, C, A, D
- CC, B, A, D
- DC, B, D, A
Correct answer
C. C, B, A, D
Step-by-step solution
The isolation of genetic material (DNA) from a fungal cell involves the following sequential steps:
First, the fungal cell wall must be broken to release the cellular contents. Since the fungal cell wall is composed of chitin, the enzyme chitinase is used to break open the cell (Step C).
Second, the cellular extract contains macromolecules such as RNA, proteins, lipids, and carbohydrates along with DNA. To obtain purified DNA, the extract is treated with specific enzymes like RNase to remove RNA and Protease to remove proteins (Step B).
Third, once the DNA is purified from other macromolecules, chilled ethanol is added to the mixture. This decreases the solubility of DNA, causing it to precipitate out of the solution (Step A).
Finally, the precipitated DNA, which appears as a collection of fine threads in the suspension, is extracted by a technique known as spooling (Step D).
Thus, the correct sequence is C, B, A, D.
Answer: C, B, A, D
First, the fungal cell wall must be broken to release the cellular contents. Since the fungal cell wall is composed of chitin, the enzyme chitinase is used to break open the cell (Step C).
Second, the cellular extract contains macromolecules such as RNA, proteins, lipids, and carbohydrates along with DNA. To obtain purified DNA, the extract is treated with specific enzymes like RNase to remove RNA and Protease to remove proteins (Step B).
Third, once the DNA is purified from other macromolecules, chilled ethanol is added to the mixture. This decreases the solubility of DNA, causing it to precipitate out of the solution (Step A).
Finally, the precipitated DNA, which appears as a collection of fine threads in the suspension, is extracted by a technique known as spooling (Step D).
Thus, the correct sequence is C, B, A, D.
Answer: C, B, A, D