Given below are the key steps involved in the isolation of genetic material from a fungal cell. Arrange these steps in the correct sequence. A. Addition of chilled ethanol to the purified mixture. B. Treatment of the cellular extract with RNase and Protease. C. Breaking open the cell using the enzyme chitinase. D. Collection of the precipitated DNA threads by spooling. Choose the correct answer from the options given

Step-by-step solution

The isolation of genetic material (DNA) from a fungal cell involves the following sequential steps: First, the fungal cell wall must be broken to release the cellular contents. Since the fungal cell wall is composed of chitin, the enzyme chitinase is used to break open the cell (Step C). Second, the cellular extract contains macromolecules such as RNA, proteins, lipids, and carbohydrates along with DNA. To obtain purified DNA, the extract is treated with specific enzymes like RNase to remove RNA and Protease to rem