NEET2026BiologyChapterActual
In the pBR322 cloning vector, the recognition sites for the restriction enzymes Pvu I and Pst I are located within which of the following regions?
Options
- AAmpicillin resistance gene ( amp^R )
- BTetracycline resistance gene ( tet^R )
- COrigin of replication ( ori )
- DGene coding for the proteins involved in replication of the plasmid ( rop )
Correct answer
A. Ampicillin resistance gene ( amp^R )
Step-by-step solution
In the pBR322 cloning vector, the ampicillin resistance gene ( amp^R ) contains the recognition sites for the restriction enzymes Pvu I and Pst I. The tetracycline resistance gene ( tet^R ) contains the recognition sites for BamH I and Sal I. The rop gene contains the recognition site for Pvu II. Therefore, Pvu I and Pst I are located within the ampicillin resistance gene. Answer: Ampicillin resistance gene ( amp^R )