NEET 2026
- Exam
- NEET
- Year
- 2026
- Subject
- Biology
- Chapter
- Chapter
NEET 2026 · Biology · Chapter
In the pBR322 cloning vector, the recognition sites for the...
In the pBR322 cloning vector, the recognition sites for the restriction enzymes Pvu I and Pst I are located within which of the following regions?
Options
- AAmpicillin resistance gene ()
- BTetracycline resistance gene ()
- COrigin of replication (ori)
- DGene coding for the proteins involved in replication of the plasmid (rop)
Correct answer
A. Ampicillin resistance gene ()
Step-by-step solution
In the pBR322 cloning vector, the ampicillin resistance gene () contains the recognition sites for the restriction enzymes Pvu I and Pst I.
The tetracycline resistance gene () contains the recognition sites for BamH I and Sal I.
The rop gene contains the recognition site for Pvu II.
Therefore, Pvu I and Pst I are located within the ampicillin resistance gene.
Answer: Ampicillin resistance gene ()
The tetracycline resistance gene () contains the recognition sites for BamH I and Sal I.
The rop gene contains the recognition site for Pvu II.
Therefore, Pvu I and Pst I are located within the ampicillin resistance gene.
Answer: Ampicillin resistance gene ()