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NEET2026BiologyChapterActual

In a particular strain of E. coli , a mutation occurs in the i gene such that the synthesized repressor protein completely loses its affinity for the operator region. Which of the following best describes the expression of the lac operon in this mutant strain?

Options

  1. ATranscription of z, y and a genes will occur only when lactose is provided in the medium.
  2. BThe operon will be transcribed continuously irrespective of the presence or absence of lactose.
  3. CThe operon will remain permanently switched off even if the inducer is added.
  4. DOnly the z gene will be transcribed while y and a genes will remain repressed.

Correct answer

B. The operon will be transcribed continuously irrespective of the presence or absence of lactose.

Step-by-step solution

The i gene of the lac operon codes for the repressor protein. Normally, this repressor binds to the operator region and prevents RNA polymerase from transcribing the structural genes ( z , y , and a ). In the given mutant strain, the repressor protein loses its affinity for the operator region. As a result, it can no longer bind to the operator to block transcription. Since the operator is never blocked by the repressor, RNA polymerase will continuously bind to the promoter and transcribe the structural genes. This

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