NEET2026BiologyChapterActual
Which of the following statements correctly describes an essential feature of an ideal cloning vector?
Options
- AIt should possess multiple recognition sites for a specific restriction enzyme to increase the chances of succ
- BThe origin of replication (ori) is a sequence that solely initiates replication but has no role in determining
- CGenes encoding resistance to antibiotics such as ampicillin or tetracycline serve as useful selectable markers
- DVectors with a very large molecular weight are generally preferred over smaller vectors to ensure stability.
Correct answer
C. Genes encoding resistance to antibiotics such as ampicillin or tetracycline serve as useful selectable markers
Step-by-step solution
An ideal cloning vector should preferably have a single recognition site for a commonly used restriction enzyme. Multiple recognition sites would generate several fragments, complicating gene cloning. The origin of replication (ori) is responsible for initiating replication and also controls the copy number of the linked DNA. Selectable markers are essential to identify and eliminate non-transformants and selectively permit the growth of transformants. Genes encoding resistance to antibiotics such as ampicillin, ch