NEET 2026
- Exam
- NEET
- Year
- 2026
- Subject
- Biology
- Chapter
- Chapter
NEET 2026 · Biology · Chapter
In order to amplify a gene of interest in vitro using the...
In order to amplify a gene of interest in vitro using the Polymerase Chain Reaction (PCR), which of the following sets of components is strictly required?
Options
- ADNA ligase, two sets of primers, and ribonucleotides
- BThermostable DNA polymerase, two sets of primers, and deoxyribonucleotides
- CRNA polymerase, one set of primers, and deoxyribonucleotides
- DRestriction endonuclease, two sets of primers, and ribonucleotides
Correct answer
B. Thermostable DNA polymerase, two sets of primers, and deoxyribonucleotides
Step-by-step solution
Polymerase Chain Reaction (PCR) is an in vitro method for amplifying a specific DNA sequence. The essential components required for PCR are:
1. A DNA template containing the gene of interest.
2. Two sets of primers (forward and reverse) that are complementary to the 3' ends of each of the two strands of the DNA segment to be amplified.
3. A thermostable DNA polymerase (such as Taq polymerase) which can withstand the high temperatures required for denaturation.
4. Deoxyribonucleotides (dNTPs), which are the building blocks used by the DNA polymerase to synthesize the new DNA strands.
DNA ligase, RNA polymerase, restriction endonucleases, and ribonucleotides are not used in standard PCR.
Answer: Thermostable DNA polymerase, two sets of primers, and deoxyribonucleotides
1. A DNA template containing the gene of interest.
2. Two sets of primers (forward and reverse) that are complementary to the 3' ends of each of the two strands of the DNA segment to be amplified.
3. A thermostable DNA polymerase (such as Taq polymerase) which can withstand the high temperatures required for denaturation.
4. Deoxyribonucleotides (dNTPs), which are the building blocks used by the DNA polymerase to synthesize the new DNA strands.
DNA ligase, RNA polymerase, restriction endonucleases, and ribonucleotides are not used in standard PCR.
Answer: Thermostable DNA polymerase, two sets of primers, and deoxyribonucleotides