NEET2026BiologyChapterActual
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A) : In recombinant DNA technology, it is essential to cut both the vector DNA and the source DNA with the same restriction endonuclease. Reason (R) : Cutting both DNA molecules with the same restriction enzyme produces complementary sticky ends that facilitate their joining by DNA ligase. In the ligh
Options
- ABoth (A) and (R) are correct and (R) is the correct explanation of (A)
- BBoth (A) and (R) are correct but (R) is not the correct explanation of (A)
- C(A) is correct but (R) is not correct
- D(A) is not correct but (R) is correct
Correct answer
A. Both (A) and (R) are correct and (R) is the correct explanation of (A)
Step-by-step solution
In recombinant DNA technology, the vector DNA and the source DNA must be cut with the same restriction endonuclease. This ensures that both DNA molecules have complementary sticky ends. The complementary sticky ends can then base-pair with each other and are subsequently joined by the enzyme DNA ligase to form a recombinant DNA molecule. Thus, both Assertion (A) and Reason (R) are correct, and (R) is the correct explanation of (A). Answer: Both (A) and (R) are correct and (R) is the correct explanation of (A)