NEET2026BiologyChapterActual
During agarose gel electrophoresis, the DNA fragments resolve and move through the matrix. The distance travelled by a specific DNA fragment towards the anode primarily depends on its:
Options
- ASequence of base pairs
- BSize of the fragment
- CRatio of purines to pyrimidines
- DType of restriction enzyme used for cleavage
Correct answer
B. Size of the fragment
Step-by-step solution
DNA fragments are negatively charged molecules, so they move towards the positive electrode (anode) under an electric field. The agarose gel matrix acts as a sieve, allowing smaller DNA fragments to move faster and further than larger ones. Therefore, the distance travelled by a specific DNA fragment during agarose gel electrophoresis primarily depends on the size of the fragment. Answer: Size of the fragment