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NEET2026BiologyChapterActual

A molecular biologist wants to isolate the genetic material from a culture of Bacillus thuringiensis . Which of the following enzymes should be used to treat the cells initially in order to break open the cell envelope?

Options

  1. ACellulase
  2. BChitinase
  3. CLysozyme
  4. DRibonuclease

Correct answer

C. Lysozyme

Step-by-step solution

Bacillus thuringiensis is a bacterium. The cell wall of bacteria is primarily composed of peptidoglycan. Lysozyme is the enzyme that specifically degrades peptidoglycan, breaking open the bacterial cell envelope to release the genetic material. Cellulase is used for plant cells, chitinase for fungal cells, and ribonuclease is used to degrade RNA, not to break open the cell. Answer: Lysozyme

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