During a forensic investigation, a scientist performs DNA profiling. Arrange the following laboratory procedures in the correct chronological order: A. Southern blotting of the resolved DNA fragments onto a nitrocellulose filter. B. Cleavage of the purified genomic DNA using restriction enzymes. C. Visualization of the band pattern using autoradiography. D. Separation of the DNA fragments under the influence of an el

Step-by-step solution

The steps of DNA fingerprinting are as follows: First, the purified genomic DNA is cleaved into fragments using restriction endonucleases (B). Next, the DNA fragments are separated based on their size using gel electrophoresis under the influence of an electric field (D). The separated DNA fragments are then transferred from the gel to a synthetic membrane such as nitrocellulose or nylon, a process known as Southern blotting (A). The membrane is then incubated with a radioactive VNTR probe, which hybridizes to spec