Arrange the following steps of recombinant DNA technology in a correct sequence. A. Ligation of the DNA fragment into a vector. B. Isolation of the desired DNA fragment. C. Transferring the recombinant DNA into the host. D. Fragmentation of DNA by restriction endonucleases. E. Culturing the host cells in a medium at large scale. Choose the correct answer from the options given below:

Step-by-step solution

Recombinant DNA technology involves several steps in a specific sequence. First, the DNA is fragmented by restriction endonucleases to cut the DNA at specific locations (Step D). Next, the desired DNA fragment is isolated from the fragmented DNA using techniques like gel electrophoresis (Step B). The isolated desired DNA fragment is then ligated into a suitable vector using the enzyme DNA ligase to form recombinant DNA (Step A). The recombinant DNA is then transferred into a suitable host cell (Step C). Finally, th