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NEET2026BiologyChapterActual

Read the following statements regarding the enzymes used in recombinant DNA technology: A. Restriction endonucleases cut the sugar-phosphate backbone of both DNA strands at specific points. B. Eco RI cuts the DNA between bases C and T only when the sequence GAATTC is present in the DNA. C. The Roman numbers following the name of a restriction enzyme indicate the order in which the enzyme was isolated from that strain

Options

  1. AA and C only
  2. BB and D only
  3. CA and D only
  4. DB and C only

Correct answer

A. A and C only

Step-by-step solution

Statement A is correct. Restriction endonucleases function by inspecting the length of a DNA sequence and binding to specific recognition sequences, where they cut the sugar-phosphate backbone of both DNA strands at specific points. Statement B is incorrect. The restriction enzyme Eco RI recognizes the palindromic sequence GAATTC and cuts the DNA between the bases G and A, not between C and T. Statement C is correct. In the nomenclature of restriction enzymes, the Roman numerals following the name indicate the orde

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