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MHT CET 202625 AprilMorning Shift
Exam
MHT CET
Year
2026
Date
25 April
Shift
Morning Shift
Subject
Biology
Chapter
Molecular Basis of Inheritance

MHT CET 2026 · 25 April · Morning Shift · Biology · Molecular Basis of Inheritance

In their experiment to prove DNA replication is semi-conservative,...

In their experiment to prove DNA replication is semi-conservative, Meselson and Stahl used 6M CsCl\text{CsCl} because the density gradient value of 6M CsCl\text{CsCl} is ________ the density gradient value of 15N^{15}\text{N} DNA.

Options

  1. Aalmost equal to
  2. Bdouble
  3. Chalf
  4. Done fourth to

Correct answer

A. almost equal to

Step-by-step solution

Meselson and Stahl used heavy isotope 15N^{15}\text{N} to label DNA and separated the heavy DNA from normal DNA using Cesium Chloride (CsCl\text{CsCl}) density gradient centrifugation.

The density of 6M CsCl\text{CsCl} is approximately 1.7 g/cm31.7 \text{ g/cm}^3, which is almost equal to the buoyant density of DNA.

During centrifugation, a density gradient is established, and DNA molecules move to the position in the gradient where the density of the CsCl\text{CsCl} solution exactly matches their own density. Therefore, 6M CsCl\text{CsCl} was chosen because its density gradient value is almost equal to the density gradient value of 15N^{15}\text{N} DNA.

Answer: almost equal to
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