MHT CET Medical202624 April 2026Evening ShiftBiologyMolecular Basis of InheritanceActual
Which of the following step in DNA fingerprinting involves the movement of negatively charged DNA fragments to the positive pole?
Options
- ASouthern blotting.
- BAutoradiography.
- CGel electrophoresis.
- DRestriction digestion.
Correct answer
C. Gel electrophoresis.
Step-by-step solution
DNA fragments are negatively charged molecules due to the presence of phosphate groups in their sugar-phosphate backbone. During gel electrophoresis, an electric field is applied across the agarose gel matrix. Under the influence of this electric field, the negatively charged DNA fragments are forced to move towards the positive electrode (anode). The fragments separate according to their size through the sieving effect of the agarose gel, with smaller fragments moving faster and further than larger ones. Southern