MHT CET 202624 AprilEvening Shift
- Exam
- MHT CET
- Year
- 2026
- Date
- 24 April
- Shift
- Evening Shift
- Subject
- Biology
- Chapter
- Molecular Basis of Inheritance
MHT CET 2026 · 24 April · Evening Shift · Biology · Molecular Basis of Inheritance
Which of the following step in DNA fingerprinting involves the...
Which of the following step in DNA fingerprinting involves the movement of negatively charged DNA fragments to the positive pole?
Options
- ASouthern blotting.
- BAutoradiography.
- CGel electrophoresis.
- DRestriction digestion.
Correct answer
C. Gel electrophoresis.
Step-by-step solution
DNA fragments are negatively charged molecules due to the presence of phosphate groups in their sugar-phosphate backbone.
During gel electrophoresis, an electric field is applied across the agarose gel matrix.
Under the influence of this electric field, the negatively charged DNA fragments are forced to move towards the positive electrode (anode).
The fragments separate according to their size through the sieving effect of the agarose gel, with smaller fragments moving faster and further than larger ones.
Southern blotting involves the transfer of separated DNA fragments from the gel to a synthetic membrane.
Autoradiography is used to detect the hybridized radioactive probes on an X-ray film.
Restriction digestion is the process of cutting DNA into smaller fragments using restriction endonucleases.
Therefore, gel electrophoresis is the step that involves the movement of negatively charged DNA fragments to the positive pole.
Answer: Gel electrophoresis.
During gel electrophoresis, an electric field is applied across the agarose gel matrix.
Under the influence of this electric field, the negatively charged DNA fragments are forced to move towards the positive electrode (anode).
The fragments separate according to their size through the sieving effect of the agarose gel, with smaller fragments moving faster and further than larger ones.
Southern blotting involves the transfer of separated DNA fragments from the gel to a synthetic membrane.
Autoradiography is used to detect the hybridized radioactive probes on an X-ray film.
Restriction digestion is the process of cutting DNA into smaller fragments using restriction endonucleases.
Therefore, gel electrophoresis is the step that involves the movement of negatively charged DNA fragments to the positive pole.
Answer: Gel electrophoresis.