MHT CET 202621 AprilEvening Shift
- Exam
- MHT CET
- Year
- 2026
- Date
- 21 April
- Shift
- Evening Shift
- Subject
- Biology
- Chapter
- Molecular Basis of Inheritance
MHT CET 2026 · 21 April · Evening Shift · Biology · Molecular Basis of Inheritance
Autoradiography in DNA fingerprinting is used to--
Autoradiography in DNA fingerprinting is used to--
Options
- ADetect hybridized DNA fragments using radioactive probes
- BCut DNA into fragments
- CAmplify DNA
- DStain the DNA
Correct answer
A. Detect hybridized DNA fragments using radioactive probes
Step-by-step solution
In DNA fingerprinting, after the DNA fragments are separated by gel electrophoresis and transferred to a nitrocellulose membrane (Southern blotting), they are hybridized with radioactive VNTR probes.
The hybridized DNA fragments are then detected by exposing the membrane to an X-ray film. This process is known as autoradiography.
Therefore, autoradiography is used to detect hybridized DNA fragments using radioactive probes.
Answer: Detect hybridized DNA fragments using radioactive probes
The hybridized DNA fragments are then detected by exposing the membrane to an X-ray film. This process is known as autoradiography.
Therefore, autoradiography is used to detect hybridized DNA fragments using radioactive probes.
Answer: Detect hybridized DNA fragments using radioactive probes