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MHT CET Medical202621 April 2026Evening ShiftBiologyMolecular Basis of InheritanceActual

Autoradiography in DNA fingerprinting is used to--

Options

  1. ADetect hybridized DNA fragments using radioactive probes
  2. BCut DNA into fragments
  3. CAmplify DNA
  4. DStain the DNA

Correct answer

A. Detect hybridized DNA fragments using radioactive probes

Step-by-step solution

In DNA fingerprinting, after the DNA fragments are separated by gel electrophoresis and transferred to a nitrocellulose membrane (Southern blotting), they are hybridized with radioactive VNTR probes. The hybridized DNA fragments are then detected by exposing the membrane to an X-ray film. This process is known as autoradiography. Therefore, autoradiography is used to detect hybridized DNA fragments using radioactive probes. Answer: Detect hybridized DNA fragments using radioactive probes

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