MHT CET Medical202623 April 2026Morning ShiftBiologyMolecular Basis of InheritanceActual
As in rDNA technology, in DNA fingerprinting, also, all the following techniques are used EXCEPT:
Options
- ARestriction enzymes.
- BCloning vector plasmids.
- CGel electrophoresis.
- DIsolated DNA segments.
Correct answer
B. Cloning vector plasmids.
Step-by-step solution
DNA fingerprinting involves the isolation of DNA, digestion of DNA by restriction endonucleases, separation of DNA fragments by gel electrophoresis, Southern blotting, hybridisation using a labelled VNTR probe, and detection by autoradiography. Cloning vector plasmids are used in recombinant DNA (rDNA) technology to carry and replicate foreign DNA inside a host cell. They are not required or used in the process of DNA fingerprinting. Answer: Cloning vector plasmids.