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MHT CET Medical202622 April 2026Evening ShiftBiologyMolecular Basis of InheritanceActual

The DNA probe used in DNA fingerprinting consists of ____(A)____ and labeled with ____(B)____. Choose the correct options for (A) and (B) respectively.

Options

  1. A(A) unknown sequence of double-stranded DNA (B) nitrocellulose filter paper
  2. B(A) known sequence of double-stranded DNA (B) variable sequences of light and heavy chain
  3. C(A) unknown sequence of single-stranded DNA (B) sticky and blunt ends
  4. D(A) known sequence of single-stranded DNA (B) radioactive isotopes

Correct answer

D. (A) known sequence of single-stranded DNA (B) radioactive isotopes

Step-by-step solution

A DNA probe is a small, known sequence of single-stranded DNA or RNA that is complementary to the specific sequence of DNA being sought. It is labeled with a radioactive isotope (or a fluorescent tag) so that it can be easily detected after it hybridizes with its complementary target sequence on the nitrocellulose membrane during DNA fingerprinting. Therefore, (A) is a known sequence of single-stranded DNA and (B) is radioactive isotopes. Answer: (A) known sequence of single-stranded DNA (B) radioactive isotopes

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