NEET 2026
- Exam
- NEET
- Year
- 2026
- Subject
- Zoology
- Chapter
- Biotechnology - Principles and Processes
NEET 2026 · Zoology · Biotechnology - Principles and Processes
During PCR, primers bind to the DNA strands in the __________ step.
During PCR, primers bind to the DNA strands in the __________ step.
Options
- Aligation
- Bdenaturation
- Cextension
- Dannealing
Correct answer
D. annealing
Step-by-step solution
Polymerase Chain Reaction (PCR) involves three main steps:
1. Denaturation: The double-stranded DNA is heated to separate it into two single strands.
2. Annealing: The temperature is lowered to allow the oligonucleotide primers to bind (anneal) to their complementary sequences on the single-stranded DNA templates.
3. Extension: The temperature is raised, and Taq DNA polymerase synthesizes the new DNA strand by adding nucleotides to the primers.
Therefore, primers bind to the DNA strands during the annealing step.
Answer: annealing
1. Denaturation: The double-stranded DNA is heated to separate it into two single strands.
2. Annealing: The temperature is lowered to allow the oligonucleotide primers to bind (anneal) to their complementary sequences on the single-stranded DNA templates.
3. Extension: The temperature is raised, and Taq DNA polymerase synthesizes the new DNA strand by adding nucleotides to the primers.
Therefore, primers bind to the DNA strands during the annealing step.
Answer: annealing