Quantrex Quantrex AcademyJEE · NEET · NDA PYQs with solutions Open app
NEET 2025
Exam
NEET
Year
2025
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

NEET 2025 · Zoology · Biotechnology - Principles and Processes

In the above represented plasmid, an alien piece of DNA is inserted...

In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies
In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

Options

  1. AUsing ampicillin & tetracycline containing medium plate.
  2. BBlue color colonies will be selected.
  3. CWhite color colonies will be selected.
  4. DBlue color colonies grown on ampicillin plates can be selected.

Correct answer

C. White color colonies will be selected.

Step-by-step solution

The correct answer is that white-colored colonies will be selected. Since an alien piece of DNA is being inserted at EcoRI site, the gene β\beta-galactosidase present here will undergo insertional inactivation. This gene is responsible for producing blue-colored colonies, but since it has been insertionally inactivated, white colored colonies will be produced. Ampicillin and tetracycline resistance genes present in the given DNA will remain intact. Thus, the given DNA will show amp R{ }^R and tet R{ }^R.
Practice Biotechnology - Principles and Processes on Quantrex Academy →

More from Biotechnology - Principles and Processes

During PCR, primers bind to the DNA strands in the __________ step. NEET 2026Given below are two statements : one is labelled as Assertion A and the other is labelled… NEET 2026Which of the following statements are not true regarding restriction endonucleases ? A.… NEET 2026Which of the following statements are correct with respect to DNA separation, isolation… NEET 2026Match List I with List II : List I List II A. Genetically modified organism I.… NEET 2026Given below are two statements : Statement I: The DNA fragments extracted from gel… NEET 2025Which of the following cannot be used for direct gene transfer? AIIMS 2019Find the correct palindromic sequence for the given DNA segment. 5'ATTGCAAT3' AIIMS 2019 Full Biotechnology - Principles and Processes list All NEET PYQs