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Biotechnology - Principles and Processes — NEET UG Zoology PYQs

737 previous year questions from Biotechnology - Principles and Processes with answers and solutions. Numbered list, year tags, and one-tap solutions — built for serious JEE / NEET practice.

737 questionsZoologySolutions on every page
1

During PCR, primers bind to the DNA strands in the __________ step.

NEET 2026 Solution View → 2

Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting

NEET 2026 Solution View → 3

Which of the following statements are not true regarding restriction endonucleases ? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the

NEET 2026 Solution View → 4

Which of the following statements are correct with respect to DNA separation, isolation and visualization ? A. The cutting of DNA is done by molecular scissors. B. The DNA fragment

NEET 2026 Solution View → 5

Match List I with List II : List I List II A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aqu

NEET 2026 Solution View → 6

In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

NEET 2025 Solution View → 7

Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA f

NEET 2025 Solution View → 8

Which of the following cannot be used for direct gene transfer?

AIIMS 2019 Solution View → 9

Find the correct palindromic sequence for the given DNA segment. 5'ATTGCAAT3'

AIIMS 2019 Solution View → 10

Select the correctly matched pair.

AIIMS 2019 Solution View → 11

Genetic variation cannot be effectively brought about by

JIPMER 2019 Solution View → 12

Which is also called molecular glue

JIPMER 2019 Solution View → 13

Which of the following statements is not correct regarding EcoRI restriction endonuclease enzyme?

AIIMS 2018 Solution View → 14

Gene transfer is present in

JIPMER 2018 Solution View → 15

Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : Stickiness of the ends facilitates the action of the enzyme DNA polymerase.

AIIMS 2017 Solution View → 16

When plants/animals are infected with virus, they may produce an anti-viral substance that is capable of inhibiting the multiplication of that virus. Identify the correct name of t

AIIMS 2017 Solution View → 17

Which of the following DNA sequences qualifies to be designated as a palindrome?

JIPMER 2017 Solution View → 18

Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the options given. A. Electrophoresis of DNA fragments B. Hybridisation with DNA probe

AIIMS 2016 Solution View → 19

Which one of the following is a recognisation site for restriction enzyme Bam HI?

JIPMER 2016 Solution View → 20

Plasmid vector in DNA recombinant technology means

AIIMS 2015 Solution View → 21

Select the correct order of processing of PCR.

AIIMS 2015 Solution View → 22

Assertion : In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeasts (eukaryotes). Reason : Both bacteria and yeasts multiply very fas

AIIMS 2015 Solution View → 23

Which of the following statements is false regarding SDS-polyacrylamide gel electrophoresis?

JIPMER 2015 Solution View → 24

Which of the following is the Pribnow box?

JIPMER 2015 Solution View → 25

How are transformants selected from non-transformants?

AIIMS 2014 Solution View → 26

Which of the following is not a characteristic of pBR322 vector?

AIIMS 2014 Solution View → 27

The vector for T-DNA is

JIPMER 2014 Solution View → 28

Which statement is correct for bacterial transduction?

JIPMER 2014 Solution View → 29

The mobile genetic element is

JIPMER 2014 Solution View → 30

In recombinant DNA technique, the term vector refers to

JIPMER 2014 Solution View → 31

Given below is a sample of portion of DNA strand given the base sequence on the opposite strand. What is so special show in it? aligned & 5'- GAATTC...3' & 3'- CTTAAG ... 5' aligne

JIPMER 2014 Solution View → 32

Which of the following is a palindromic sequence?

AIIMS 2013 Solution View → 33

Which of the following is a cloning vector?

AIIMS 2013 Solution View → 34

The thermostable enzymes, Taq and Pfu, isolated from thermophilic bacteria are

JIPMER 2013 Solution View → 35

Biolistic technique is used in

JIPMER 2013 Solution View → 36

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

NEET 2013 Solution View → 37

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

NEET 2013 Solution View → 38

Gel electrophoresis is used for

JIPMER 2012 Solution View → 39

Which one is a true statement regarding DNA polymerase used in PCR?

NEET 2012 Solution View → 40

For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of

NEET 2012 Solution View → 41

The figure below is the diagrammatic representation of the E . coli vector pBR 322 . Which one of the given options correctly identifies its certain components (s)?

NEET 2012 Solution View → 42

Removal of RNA polymerase III from nucleoplasm will affect the synthesis of

NEET 2012 Solution View → 43

A single strand of nucleic acid tagged with a radioactive molecule is called

NEET 2012 Solution View → 44

Which one of the following represents a palindromic sequence in DNA?

NEET 2012 Solution View → 45

Which one of the following is a case of wrong matching?

NEET 2012 Solution View → 46

In genetic engineering, the antibiotics are used

NEET 2012 Solution View → 47

The figure below shows three steps (I, II, III) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?

NEET 2012 Solution View → 48

Biolistics (gene-gun) is suitable for

NEET 2012 Solution View → 49

What is the source of EcoRI?

AIIMS 2011 Solution View → 50

A kind of biotechnology involving manipulation of DNA is

JIPMER 2011 Solution View → 51

Given below is a sample of portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it? 5^ _ GAATTC __ 3^ 3 - CTTAAG 5^

NEET 2011 Solution View → 52

Agarose extracted from sea weeds finds use in

NEET 2011 Solution View → 53

Which one of the following also acts as a catalyst in a bacterial cell?

NEET 2011 Solution View → 54

Read the following four statements (I-IV) about certain mistakes in two of them I. the first transgenic buffalo, Rosie produced milk which was human alpha-lactal bumin enriched. II

NEET 2011 Solution View → 55

There is a restriction endonuclease called E c o RI. What does 'co' part in it stand for?

NEET 2011 Solution View → 56

What is a plasmid?

AIIMS 2010 Solution View → 57

Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the break down of fungal cell, plant c

AIIMS 2010 Solution View → 58

The process which can not take place in the absence of viruses is

JIPMER 2010 Solution View → 59

Stirred-tank bioreactors have been designed for

NEET 2010 Solution View → 60

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

NEET 2010 Solution View → 61

DNA or RNA segment tagged with a radioactive molecule is called

NEET 2010 Solution View → 62

Genetic engineering has been successfully used for producing

NEET 2010 Solution View → 63

Which of the following is used in gene cloning?

NEET 2010 Solution View → 64

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the c

NEET 2010 Solution View → 65

Which one of the following is used as vector for cloning genes into higher organisms?

NEET 2010 Solution View → 66

Restriction endonucleases are enzymes which

NEET 2010 Solution View → 67

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.

AIIMS 2009 Solution View → 68

Polyethylene glycol method is used for

NEET 2009 Solution View → 69

Which one of the following pairs is wrongly matched?

NEET 2009 Solution View → 70

Which one of the following is commonly used in transfer of foreign DNA into crop plants ?

NEET 2009 Solution View → 71

Polyethylene glycol method is used for :

NEET 2009 Solution View → 72

Assertion : Hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesized by myeloma cells. Reason : This medium allows selection of hybridoma cells.

AIIMS 2008 Solution View → 73

Hamburger shift is also known as

JIPMER 2008 Solution View → 74

Which of the following is used as a best genetic vector in plants ?

JIPMER 2008 Solution View → 75

Modern detergents contain enzyme preparations of

NEET 2008 Solution View → 76

Gel electrophoresis is used for

NEET 2008 Solution View → 77

The linking of antibiotic resistance gene with the plasmid vector became possible with

NEET 2008 Solution View → 78

Gel electrophoresis is used for

NEET 2008 Solution View → 79

The linking of antibiotic resistance gene with the plasmid vector became possible with

NEET 2008 Solution View → 80

Which one of the following hydrolysis internal phosphodiester bonds in a polynucleotide chain?

JIPMER 2007 Solution View → 81

Two microbes found to be very useful in genetic engineering are:

NEET 2006 Solution View → 82

Restriction endonuclease:

NEET 2006 Solution View → 83

Assertion : In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote). Reason : Both bacteria and yeast multiply very fast t

AIIMS 2005 Solution View → 84

Production of a human protein in bacteria by genetic engineering is possible because:

NEET 2005 Solution View → 85

What is the firststep in the Southern Blottechnique?

AIIMS 2004 Solution View → 86

An example of gene therapy is

AIIMS 2004 Solution View → 87

Which of the following pairs is correctly matched?

JIPMER 2004 Solution View → 88

Micro-organism which act as a vehicle for foreign gene transfer in higher plants:

JIPMER 2004 Solution View → 89

Restriction enzymes are used in genetic engineering, because they

JIPMER 2004 Solution View → 90

Restriction endonucleases:

NEET 2004 Solution View → 91

In transgenics, expression of transgene in target tissue is determined by:

NEET 2004 Solution View → 92

Restriction enzymes:

AIIMS 2003 Solution View → 93

Introduction of foreign genes for improving genotype is

AIIMS 2002 Solution View → 94

Manipulation of DNA in genetic engineering became possible due to the discovery of:

NEET 2002 Solution View → 95

Introduction of food plants developed by genetic engineering is not desirable because:

NEET 2002 Solution View → 96

Where was Herbert Boyer born and raised?

NEET Solution View → 97

Where did Herbert Boyer complete his graduate work?

NEET Solution View → 98

Where did Herbert Boyer take over assistant professorship in 1966?

NEET Solution View → 99

What was Herbert Boyer's discovery in 1969?

NEET Solution View → 100

What was the outcome of Herbert Boyer's discovery in 1969?

NEET Solution View → 101

Who did Herbert Boyer have a conversation with in Hawaii?

NEET Solution View → 102

What was Stanley Cohen studying?

NEET Solution View → 103

What was the basis upon which the discipline of biotechnology was founded?

NEET Solution View → 104

What is biotechnology?

NEET Solution View → 105

Can making curd be considered a form of biotechnology?

NEET Solution View → 106

What does the modern sense of biotechnology focus on?

NEET Solution View → 107

What is included in biotechnology according to EFB's definition?

NEET Solution View → 108

What is an example of a biotechnology process mentioned in the paragraph?

NEET Solution View → 109

What is the main difference between traditional biotechnology and modern biotechnology?

NEET Solution View → 110

What is the definition of biotechnology given by the European Federation of Biotechnology (EFB)?

NEET Solution View → 111

Can biotechnology be used to correct a defective gene?

NEET Solution View → 112

What are the two core techniques that enabled the birth of modern biotechnology?

NEET Solution View → 113

.What does genetic engineering involve?

NEET Solution View → 114

What is the main purpose of bioprocess engineering?

NEET Solution View → 115

What is the outcome of bioprocess engineering?

NEET Solution View → 116

What are some of the products that bioprocess engineering can manufacture?

NEET Solution View → 117

What is the difference between genetic engineering and bioprocess engineering?

NEET Solution View → 118

What is the advantage of sexual reproduction over asexual reproduction?

NEET Solution View → 119

What is the main goal of genetic engineering?

NEET Solution View → 120

What is the likely fate of a piece of DNA transferred into an alien organism?

NEET Solution View →
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