An analysis of chromosomal DNA using the Southern hybrization technique does not use:

Step-by-step solution

The method is named after its inventor, the British biologist Edwin Southern. Southern blotting is a hybridization technique that enables researchers to determine the presence of certain nucleotide sequences in a sample of DNA. This technique involves the following steps: 1. DNA is digested with one or more restriction enzymes, and the resulting fragments are separated according to size by electrophoresis through agarose or polyacrylamide gel. 2. The DNA is denatured and transferred from the gel to a solid support,