NEST2019BiologyBiotechnology and Its Applications
A transgenic crop plant (2 n ) was generated by transforming it with a kanamycinresistant gene ' P ' that integrates in its nuclear genome. Positive transformants were screened using this antibiotic as a selective agent. Southern blot analysis revealed a single copy insertion. Which of the following statement(s) is/are correct?
Options
- AP /- transgenic line can have stronger expression level of gene 'P' than the P / P transgenic line.
- BIf the transformants are selfed, the offspring will have either P / P , P /- or -/- genotypes.
- CThe Southern analysis band pattern for P / P and P /- will not be the same.
- DP / P , P /- genotype plants should survive the selection.
Correct answer
D. P / P , P /- genotype plants should survive the selection.
Step-by-step solution
No solution available.