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NEST2013BiologyBiotechnology and Its Applications

Agrobacterium-mediated transformation is routinely used for the development of transgenic plants. In these experiments, a suitable plant part (explant) is incubated with Agrobacterium cells containing the gene of interest (passenger gene) that is to be introduced into the plant. In addition, a selectable marker gene is also used to ensure growth of only the transformed cells on regeneration media containing the selec

Options

  1. ARegeneration medium for use of the selection marker in construct II was not optimised.
  2. BExpression of the D E P gene is lethal to the transformed cells.
  3. CDegradation of Hygromycin occurred in experiments with construct II.
  4. DThe H Y G^R gene used in construct II lost its functionality probably due to mutation(s) either in the gene or

Correct answer

D. The H Y G^R gene used in construct II lost its functionality probably due to mutation(s) either in the gene or

Step-by-step solution

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