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What was the first instance of recombinant DNA construction made of?

Options

  1. AA piece of cut DNA from a plasmid responsible for antibiotic resistance
  2. BA type of bacterium
  3. CA viral particle
  4. DAntibiotic resistance gene

Correct answer

A. A piece of cut DNA from a plasmid responsible for antibiotic resistance

Step-by-step solution

The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid (autonomously replicating circular extra-chromosomal DNA) of Salmonella typhimurium. Stanley Cohen and Herbert Boyer accomplished this in 1972 by isolating the antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance

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