NEETZoologyBiotechnology - Principles and Processes
How does Hind II cut DNA molecules?
Options
- ABy recognising a specific sequence of base pairs
- BBy adding methyl groups to DNA
- CBy restricting the growth of bacteriophage
- DNone of the above
Correct answer
A. By recognising a specific sequence of base pairs
Step-by-step solution
The first restriction endonuclease–Hind II, whose functioning depended on a specific DNA nucleotide sequence was isolated and characterized five years later. It was found that Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of six base pairs . This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzymes that have been isolated from over 230 strains of bacteria each of which recognize different