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What is the function of agarose gel in gel electrophoresis?

Options

  1. ATo separate DNA fragments based on their size
  2. BTo form recombinant vector molecule
  3. CTo create hydrogen bonds
  4. Dact as a medium

Correct answer

D. act as a medium

Step-by-step solution

The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since >DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. Nowadays the most commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate (resolve) according to their size through sieving effect provide

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