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What is the function of DNA ligase?

Options

  1. ATo join the sticky ends of DNA fragments
  2. BTo visualize the separated DNA fragments
  3. CTo separate the fragments of DNA
  4. DTo form recombinant molecules of DNA

Correct answer

A. To join the sticky ends of DNA fragments

Step-by-step solution

Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. Restriction endonucleases are used in genetic engineering to form ‘recombinant

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