NEETZoologyBiotechnology - Principles and Processes
Why is it important to have a high copy number of bacteriophage or plasmid genome in a cell?
Options
- AIt makes it easy to link foreign DNA
- BIt facilitates selection of non-recombinants
- CIt reduces the number of copies of the genome
- DIt does not have any importance
Correct answer
A. It makes it easy to link foreign DNA
Step-by-step solution
You know that plasmids and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA. Bacteriophages because of their high number per cell, have very high copy numbers of their genome within the bacterial cells. Some plasmids may have only one or two copies per cell whereas others may have 15-100 copies per cell. Their numbers can go even higher. If we are able to link an alien piece of DNA with bacteriophage or plasmid DNA, we can multiply its numbers equal t