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What is the function of a selectable marker in transformation?

Options

  1. ATo identify and eliminate nontransformants
  2. BTo support high copy number
  3. CTo start replication
  4. DTo permit the growth of transformants

Correct answer

A. To identify and eliminate nontransformants

Step-by-step solution

In addition to ‘ori’, the vector requires a selectable marker, which helps in identifying and eliminating nontransformants and selectively permitting the growth of the >transformants. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium (you will study the process in subsequent section). Normally, the genes encoding >resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. The normal

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