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What is the result of inserting a recombinant DNA within the coding sequence of an enzyme, β-galactosidase?

Options

  1. AThe colonies produce blue colour
  2. BThe colonies do not produce any colour
  3. CThe colonies produce red colour
  4. DThe colonies produce yellow colour

Correct answer

B. The colonies do not produce any colour

Step-by-step solution

Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneous plating on two plates having different antibiotics. Therefore, alternative selectable markers have been developed which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, β-galactosidase. This results into inactivation of the gene

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