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NEET
Exam
NEET
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

NEET · Zoology · Biotechnology - Principles and Processes

How is heat shock used in introducing recombinant DNA into bacteria?

How is heat shock used in introducing recombinant DNA into bacteria?

Options

  1. ABy injecting DNA directly into the nucleus
  2. Bbombarding cells with high velocity micro-particles
  3. CBy allowing a disarmed pathogen to infect the cell
  4. DBy incubating the cells with recombinant DNA on ice and then placing them briefly at 42°C

Correct answer

D. By incubating the cells with recombinant DNA on ice and then placing them briefly at 42°C

Step-by-step solution

Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 420C (heat shock), and then putting them back on ice. This enables the bacteria to take up the recombinant DNA.

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