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What is the purpose of breaking the cell open?

Options

  1. ATo extract the genetic material
  2. BTo add restriction enzymes
  3. CTo remove the RNA
  4. DTo remove the proteins

Correct answer

A. To extract the genetic material

Step-by-step solution

Recall that nucleic acid is the genetic material of all organisms without exception. In majority of organisms this is deoxyribonucleic acid or DNA. In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macro-molecules. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids . This can be achieved by treating the bacterial cells/plant or animal tissue w

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