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What is the function of ligase in restriction enzyme digestion and ligation process?

Options

  1. ATo cut the source DNA
  2. BTo cut the vector DNA
  3. CTo prepare recombinant DNA
  4. DTo purify the DNA

Correct answer

C. To prepare recombinant DNA

Step-by-step solution

Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the optimal conditions for that specific enzyme. Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion. DNA is a negatively charged molecule, hence it moves towards the positive electrode (anode). The process is repeated with the vector DNA also. The joining of DNA involves several processes. After having cut the source DNA as well as the vector DNA with a spe

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