NEETZoologyBiotechnology - Principles and Processes
How many copies of a segment of DNA can be produced in PCR?
Options
- A1 million
- B10 million
- C100 million
- D1 billion
Correct answer
D. 1 billion
Step-by-step solution
PCR stands for Polymerase Chain Reaction. In this reaction, multiple copies of the gene (or DNA) of interest is synthesised in vitro using two sets of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. The enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template . If the process of replication of DNA is repeated many times, the segment of DNA can be amplified to approximately billion tim