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What is the purpose of using a thermostable DNA polymerase in PCR?

Options

  1. ATo degrade the DNA
  2. BTo extend the primers
  3. CTo remain active during the high temperature induced denaturation of double stranded DNA
  4. DTo amplify the DNA

Correct answer

C. To remain active during the high temperature induced denaturation of double stranded DNA

Step-by-step solution

PCR stands for Polymerase Chain Reaction. In this reaction, multiple copies of the gene (or DNA) of interest is synthesised in vitro using two sets of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. The enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template. If the process of replication of DNA is repeated many times, the segment of DNA can be amplified to approximately billion time

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