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What is the purpose of introducing ligated DNA into recipient cells?

Options

  1. ATo increase the number of antibiotic-resistant cells
  2. BTo study the behavior of transformed cells
  3. CTo clone genes of interest
  4. DTo produce recombinant DNA

Correct answer

C. To clone genes of interest

Step-by-step solution

PCR stands for Polymerase Chain Reaction. In this reaction , multiple copies of the gene (or DNA) of interest is synthesised in vitro using two sets of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. The enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template. If the process of replication of DNA is repeated many times, the segment of DNA can be amplified to approximately billion tim

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