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What is the role of agar plates in recombinant technologies?

Options

  1. ATo allow transformed cells to grow
  2. BTo inhibit the growth of untransformed cells
  3. CTo allow the expression of foreign genes in host cells
  4. DTo purify the desired protein

Correct answer

B. To inhibit the growth of untransformed cells

Step-by-step solution

if a recombinant DNA bearing gene for resistance to an antibiotic (e.g., ampicillin) is transferred into E. coli cells, the host cells become transformed into ampicillin-resistant cells. If we spread the transformed cells on agar plates containing ampicill in, only transformants will grow, untransformed recip ient cells will die . Since, due to ampicillin resistance gene, one is able to select a transformed cell in the presence of ampicillin. The ampicillin resistance gene in this case is called a selectable marker

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