NEETZoologyBiotechnology - Principles and Processes
The cutting of DNA at specific locations became possible with the discovery of____
Options
- ARestriction endonuclease enzymes
- BProbes
- CSelectable markers
- DLigases
Correct answer
A. Restriction endonuclease enzymes
Step-by-step solution
Stanley Cohen and Herbert Boyer accomplished this in 1972 by isolating the antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance. The cutting of DNA at specific locations became possible with the discovery of the so-called ‘molecular scissors’– restriction enzymes. The cut piece of DNA was then linked with the plasmid DNA. These plasmid DNA act as vectors to transfer the piece of DNA attached to it.