NEETZoologyBiotechnology - Principles and Processes
Restriction endonucleases are enzymes which
Options
- ARemove nucleotides from the ends of the DNA molecule
- BMake cuts at specific positions within the DNA molecule
- CRecognise a specific nucleotide sequence for binding of DNA ligase
- DRestrict the action of the enzyme DNA polymerase
Correct answer
B. Make cuts at specific positions within the DNA molecule
Step-by-step solution
Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas endonucleases make cuts at specific positions within the DNA. Each restriction endonuclease functions by ‘inspecting’ the length of a DNA sequence