NEETZoologyBiotechnology - Principles and Processes
When cut by the same restriction enzyme the resultant DNA fragments have the same kind of sticky ends and these can be joined together using
Options
- ARestriction endonuclease
- BHelicase
- Cligase
- Dpolymerase
Correct answer
C. ligase
Step-by-step solution
Restriction endonucleases are used in genetic engineering to form ‘recombinant’ molecules of DNA, which are composed of DNA from different sources/genomes. When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ‘sticky-ends’ and these can be joined together (end-to-end) using DNA ligases.You may have realised that normally, unless one cuts the vector and the source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created