NEETZoologyBiotechnology - Principles and Processes
Restriction endonucleases are enzymes that P. Cleave the 5' terminal nucleotides from duplex DNA molecules Q. Make sequence-specific cuts in both strands of duplex DNA molecules R. Promote circularisation of the duplex DNA molecule by removal of the 5' terminal nucleotides S. Break their sugar phosphate bonds which is present in DNA double helix structure
Options
- AP, Q
- BP, R
- CQ, S
- DP, Q, R, S
Correct answer
C. Q, S
Step-by-step solution
Each restriction endonuclease functions by ‘inspecting’ the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar -phosphate backbones. Each restriction endonuclease recognises a specific palindromic nucleotide sequence in the DNA.