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Imagine a gel through which DNA fragments have moved in response to an applied electrical current. The band on this gel that is farthest from the top (that is, from the place where the DNA fragments were added to the “well”) represents the

Options

  1. AShortest fragments of DNA
  2. BLongest fragments of DNA
  3. CRestriction enzyme used to cut the DNA into fragments
  4. DLigase used to bind the DNA fragments together

Correct answer

A. Shortest fragments of DNA

Step-by-step solution

Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. Nowadays the most commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. Hence, the smaller the fragment size the farther it moves.The separated DNA fragments can be visualised only after staining the DNA with a

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